Ppt infrared spectroscopy powerpoint presentation free. In the ab o v e gure ra yleigh band is sho wn in middle. The technique became widely used after the invention of the laser. It is based on periodic changes of dipolmoments ir or polarizabilities raman caused by molecular vibrations of molecules or groups of atoms and the combined discrete energy transitions and changes of frequencies during absorption ir or scattering raman of. Raman spectroscopy rayleigh and raman scattering stokes and antistokes as seen on energy level diagram. Infrared and raman spectra of inorganic and coordination. Spektroskopi ultra violet dan sinar tampak spektroskopi uv vis oleh. Ftir spectroscopy is a rapid, economical, easy, and nondestructive technique deserving wider use in clay mineral investigations. Spectroscopy also provides a precise analytical method for finding the. We shall look first at the study of free surfaces and of epitaxial growth on them where, for instance, surface reconstructions can lead to surfacespecific vibrational states which raman spectroscopy can detect, as shown in fig.
Raman spectroscopy and resonance raman spectroscopy are wellestablished techniques that are widely exploited scientifically and technologically. Written by three acknowledged experts this title uses examples to illustrate the usefulness of the technique of raman spectroscopy in such diverse areas as forensic science, biochemistry, medical, pharmaceutical prescription and illicit drugs. For any real molecule, absolute separation of the different motions is seldom encountered since molecules are simultaneously undergoing rotation and vibration. Recent advances in underlying technology, such as lasers, detectors, filters and components, have transformed the technique into a very effective modern tool for studying complex biological problems. We seek to combine competences to construct a versatile technology platform with the vision of achieving a technique, which is applicable to analysis of any sample. Ftir spectroscopy an overview sciencedirect topics. Smekal in 1923 1 and first observed experimentally in 1928 by raman and krishnan 2. Thanks to choon kiat sim fall, 2005 for this photo and parts of the writeup. An integrated raman spectroscopy and mass spectrometry. Raman spectra are usually shown in wavenumbers as a shift from the rayleigh scattered line. Since then, the phenomenon has been referred to as raman spectroscopy. The p in icp stands for plasma, an ionized gas consisting of positive ions and free electrons.
Phthalate esters as softeners in pvc plastics dtu chemistry. Raman vs ir spectroscopy raman ir sample preparation usually simpler liquid solid samples must be free from dust biological materials usually fluoresce, masking scattering spectral measurements on vibrations halide optics must be usedmade in the visible regionglass cells expensive, easily broken, may be used water soluble depolarization. Spectroscopy spectroscopy energy states of real diatomic molecules. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect. View raman spectroscopy research papers on academia. The ra yleigh band is v ery in tense as compared to the raman. Raman spectroscopy university of california, irvine. Raman spectroscopy provides a molecular fingerprint of the cellular state, a complex spectrum resulting from the contributions of many molecules inside the cell. Although the incident radiation can vary, a great deal of success was reported with a hollow cathode ion laser that produces light at 224 and 248 nm. Vibrational spectroscopy vibrational spectroscopy ir, raman. Raman chapter 18 part 11 raman spectroscopy scattering. Raman spectroscopy an overview sciencedirect topics. Analysis of phthalate ester content in pvc plastics by means of ftraman spectroscopy, applied spectroscopy, vol. Raman spectroscopy for chemical analysis request pdf.
In nature, plasmas usually form only in stars, where the temperatures are high enough to ionize the gas. In the original experiment sunlight was focussed by a telescope onto a sample which was either a purified liquid or a dust free vapour. Mos 2, moo 3 changes in frequency of raman peak stressstrai n state e. February 15, 2006 advanced physics laboratory raman. Excitation induces emission of light from the sample usually of different frequency. Raman spectroscopy information from raman spectroscopy characteristic raman frequencies composition of material e.
Infrared spectroscopy ir theory and interpretation of ir spectra assigned readings introduction to technique 25 p. Their displacemen ts from 0 giv e the frequencies of normal vibrations. The raman susceptibility is sensitive to the lipid phases and reflects the in. The raman spectrophotometer university of pennsylvania. Raman spectra of free molecules and of their inclusion complexes have been recorded in the energy ranges 1050 and 1003500 cm, and significant energy shifts, intensity variations and. Raman effect, named after its discoverer, the indian scientist c v raman raman won the nobel prize in 1930 for his discovery, accomplished using filtered sunlight as a monochromatic source of photons, a colored filter as a monochromator, and a human eye as detector. Raman spectroscopy is commonly used in chemistry to provide a structural fingerprint by which molecules can be identified. Isotope effect of the phonons mean free path in graphene by microraman. The large number of wavelengths emitted by these systems makes it possible to investigate their structures in detail, including the electron configurations of ground and various excited states. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. Spectroscopy is used as a tool for studying the structures of atoms and molecules. Raman is a spectroscopic technique typically used to determine vibrational modes of molecules, although rotational and other lowfrequency modes of systems may also be observed. Raman spectroscopy is based on scattering of radiation raman scattering, which is a phenomenon discovered in 1928 by physicist sir c.
Spesktroskopi raman pada carbon nanotubes material science. Tekuk bending inplane rocking inplane scissoring outplane wagging outplane twisting. Vibrational spectroscopy vibrational spectroscopy is an energy sensitive method. An associated spectrum is included, note the raman lines intensity are greatly exaggerated. Whilst first reported by raman himself in 1928 1 the key to wide spread use of raman spectroscopy was the development of lasers, tunable lasers in the case of resonance raman, to provide high. In situ raman cells as lein atle rmo pell rtz hip ring higte rat men r l in gwir mple wdow a p h h m w ramanscattered light gas inlet gas outlet thermocouple well quartz chips oring hightemperature cement quartz wool heating wire quartz window sample laser excitation at low laser power to minimize heating raman. Raman spectrophotometers all have the same basic components. Kalau spektroskopi uvvis menggunakan berkas cahaya ultraviolet dan cahaya tampak sebagai sumber energi untuk mengeksitasi atommolekul, spektroskopi raman menggunakan berkas cahaya monokromatis berupa laser. Resonance raman spectroscopy rr spectroscopy is a raman spectroscopy technique in which the incident photon energy is close in energy to an electronic transition of a compound or material under examination.
Raman chapter 18 part 11 free download as powerpoint presentation. Modern instruments offer high sensitivity, speedy data collection, enhanced spectral precision, and reproducibility. Spectres raman du alphaglucose vitreux obtenu par trempe du liquide et cryobroyage. The uv raman spectroscopy project is an interdisciplinary collaboration between researchers at the technical university of denmark dtu and other universities. For the last five decades raman spectroscopy has been widely utilized in biological research fields. Laser ablation inductively coupled plasma optical emission spectroscopy laicpoes has a ridiculously complicated name, so lets start with icp, which is the heart of the analytical technique. Raman spectroscopy 21506 figure 3 the raman spectrometer, which is linked by a dual fiber optic cable to the sample holder bottom right, and the sample bottles left. Positionieren des beweglichen interferometerspiegels. Resonance raman spectroscopy of extreme nanowires and. A free powerpoint ppt presentation displayed as a flash slide show on id. Vch verlagsgesellschaft, weinheim, basel, new york.
Winner of the standing ovation award for best powerpoint templates from presentations magazine. Journal of raman spectroscopy wiley online library. Sama seperti teknik spektroskopi lainnya, prinsip spektroskopi raman adalah adanya interaksi antara cahaya dan materi. All nuclei with unpaired protons or neutrons are magnetically active they have a magnetic field arising from the unpaired nuclear particle. A laser source is needed to excite the target species. This second edition of introductory raman spectroscopy serves as a guide to newcomers who wish to become acquainted with this dynamic technique. Raman spectroscopy has been known and used as a technique for 80 years, originally for the study of inorganic substances.
The spectroscopy of raman scattering, or raman spectroscopy, allows label free and quantitative molecular sensing of a biological sample in situ without disruption. To achieve this, we have designed a unique system in which living single cells are screened using label free raman spectroscopy then further characterized using mass spectrometry 4. The progress in ftir spectrometer designs has greatly enhanced the field of their applications. The field of raman spectroscopy was greatly enhanced by the advent of laser technology during the 1960s. Maywan hariono 2009 salycilic acid ethanol contoh spektrum allopurinol phenytoin references david g. Fluorescence emission from excited electronic singlet states phosphorescence emission from excited electronic triplet states raman scattering light scattering involving vibrational transition. Report spektroskopi raman please fill this form, we will try to respond as soon as possible. Spectroscopy energy states of real diatomic molecules. Raman spectroscopy is no exception because glucose naturally exhibits weak raman signals due to its small raman scattering crosssection, measuring only 5. Raman scattering of a cell conveys the intrinsic information inherent to chemical structures of biomolecules. Raman signal is a million th in tensit y of the inciden t ligh t. Of greatest interest to an organic chemist is hydrogen including deuterium and carbon the c isotope not the 12c isotope.
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